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CABRI:ICLC |
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Accession_number |
ICLC ATL98015
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Cell_line_name |
B104-1-1
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Brief_description |
Species: mouse, Swiss albino; Tissue: embryo; DNA from rat neuroblastoma B104; Original line: NIH/3T3
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Description |
Species: mouse, Swiss albino; Tissue: embryo; Transfected with EcoR1 digested DNA from the rat neuroblastoma B104; Original line: NIH/3T3
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Depositor |
Dr U. Pfeffer, Molecular Biology Dept IST at ABC, Genoa, Italy
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Bibliographic_reference |
Nature 1984;312:513-516 [PMID: 6095109
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Morphology |
fibroblast, grown as monolayer
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Culture_conditions |
continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
the cells contain the transforming gene neu which induces synthesis of a tumor antigen of relative molecular mass 185,000 (p185), strongly associated with the presence of neuro/glioblastoma oncogenes. The neu oncogene bears homology to erb-B and the tumor antigen p185 is serologically related to the epidermal growth factor receptor
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by isoenzymes: confirmed as mouse with MD, NP
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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